Corynespora cassiicola corycaoverview eppo global database. Detection of corynespora cassiicola in hevea rubber tree. A total of 651 isolates of cucumber corynespora leaf spot fungus corynespora cassiicola collected from cucumber in japan, either with 438 isolates or without 2 isolates a prior histo ry of boscalid use, were tested for their sensitivity to boscalid by using a mycelial growth inhibition method on. Whole genome sequences of four cotton and one soybean isolate from tennessee were used to develop single nucleotide polymorphism markers for cotton isolates. Cassiicola known elsewhere causing leaf spot of cotton. This fungus is present in some hevea rubber plantations and nurseries in hainan and yunnan provinces of south china. Chemical investigation of the ethyl acetate extract of corynespora cassiicola, isolated from leaf tissues of the chinese mangrove medicinal plant laguncularia racemosa, yielded four new secondary metabolites, including three decalactones, xestodecalactones df as well as corynesidone c 4, in addition to four known compounds. Structural analysis of cassiicolin, a hostselective.
The isolation ofum591 from a patients contact lens indicates the pathogenic. Cotton isolates had little diversity at the genome level and very. The molecular detection of corynespora cassiicola on. The fungus corynespora cassiicola is an important pathogen that causes necrotic lesions in several plant species. Pdf sensitivity of corynespora cassiicola from soybean to. Detection of corynespora cassiicola in soil with realtime. Frontiers genomewide analysis of corynespora cassiicola. The organism was isolated and identified as corynespora cassiicola according to its morphological characteristics and. Leaf spot of pawpaw is hereby reported for the first time in nigeria. Previous studies revealed that populations from the three hosts are genetically distinct and host specialized. Initial characterization of corynespora cassiicola affecting florida tomatoes pdf. Growth inhibition was evaluated measuring the diameter of the.
Corynespora cassiicola is causing numerous emerging diseases, including target spot of cotton a disease is emerging if increase in host range, geographic range, or incidence is. In the rubber tree, this fungus causes the corynespora leaf fall clf disease, which increasingly affects natural rubber production in asia and africa. Among them, 12, 34, 56, and 78 were pairs of epimers. Few studies referred to the formation of chlamydospores by c. Chemical investigation of the ethyl acetate extract of corynespora cassiicola, isolated from leaf tissues of the chinese mangrove medicinal plant laguncularia racemosa, yielded four new secondary. Brazil is the worlds second major producer of coffea canephora solitary or coalesce and form extensive irregular lesions. Transformation of corynespora cassiicola by agrobacterium. Structural analysis of cassiicolin, a hostselective protein toxin from corynespora cassiicola. A case of phaeohyphomycosis caused by corynespora cassiicola. A boll rot of cotton caused by corynespora cassiicola in.
Transcriptome and mirna analyses of the response to. Cca corynespora blight and target spot corynespora cassiicola gc powdery mildew golovinomyces cichoracearum ccu scab and gummosis cladosporium cucumerinum kgmmv kyuri green mottle mosaic virus cgmmv cucumber green mottle mosaic virus pcu downy mildew pseudoperonospora cubensis cmv cucumber mosaic virus prsv papaya ringspot virus. Corynespora cassiicola from cotton and soybean in the southeastern united. Metconazole exhibited excellent preventive and curative activities in vivo metconazole reduced disease severity of corynespora. An updated phylogenetic classification of corynespora. We do not use these to store personal information about you. Sobers the first report of corynespora cassiicola berk. Differentiation of corynespora cassiicola and cercospora. Chromone derivatives from a spongederived strain of the. Diagnostic features of corynespora cassiicola and its. Several lineages of this pathogen have been recognized, but the classification of isolates into clades is timeconsuming and still sometimes leads to unclear results. Documents about corynespora cassiicola coryca this website uses cookies our website uses cookies to ensure that we give you the best possible online experience.
The isolate, which on cotton is pathogenic only to bolls, is considered to be different from a strain ofc. Growth and sporulation of the fungus on several media. The isolation of um 591 from a patients contact lens indicates the pathogenic potential of this dematiaceous fungus in human. Two primer pairs showed specificity to corynespora cassiicola and one primer pair showed specificity to cercospora sp. Red, lesions enlarged, coalesced, and finally covered the entire leaves of cucumber. We report a case of subcutaneous phaeohyphomycosis caused by a plant pathogen in a patient with acute heart failure. Morphological and molecular characterization of isolates.
B hyphae with septa black arrow in the edematous papillary dermis periodic. The symptom is characterized by a papery center surrounded by a yellow halo. The causal organism was isolated in pure culture and identified ascorynespora cassiicola berk. Corynespora cassiicola corycadocuments eppo global database.
Saprophytism and parasitism habits of plants are common survival strategies for this pathogen. The organism was isolated and identified as corynespora cassiicola according to its morphological characteristics and gene. Corynespora cassiicola attackes diverse agriculturally important plants, including soybean and cotton, in the us. Phylloplane fungi % biological control % corynespora % rubber % antagonism. Diversity of the cassiicolin gene in corynespora cassiicola. Purification and characterization of cassiicolin, the toxin produced by corynespora cassiicola, causal agent of the leaf fall disease of rubber tree. Corynespora cassiicola is an important pathogen because its responsible for a considerable loss of crop production and yield. Unlimited viewing of the articlechapter pdf and any associated supplements and figures. Pathogenicity of corynespora cassiicola on different hosts. Only a single, historical clinical case of human infection caused by c. Pdf purification and characterization of cassiicolin.
In the last few decades it has become one of the most destructive foliar and fruit diseases of tomato in the state mackenzie et al. Two single nucleotide polymorphisms snps were detected in the internal transcribed spacer region of the nuclear ribosomal dna rdnaits of 21 corynespora cassiicola isolates obtained from a number of hevea clones grown in rubber plantations in. Hofs inhibit corynespora cassiicola growth in vitro and protect glycine max plants against this pathogen. Pdf infection process of corynespora cassiicola tagged.
Corynespora cassiicola is an ascomycetes fungus with a broad host range and diverse life styles. Twelve new chromone derivatives, corynechromones al 112, were isolated from the spongederived fungus corynespora cassiicola xs200900i7. Evaluation phylloplane fungi as biocontrol agent of corynespora lea. The absolute configurations of 110 were assigned by the modified moshers method and tddft ecd calculations. A new foliar disease, corynespora leaf spot, has been detected in arkansas cotton travis faske extension plant pathologist corynespora leaf spot has been detected in several commercial cotton fields on a few varieties fm 1944, st 5458, and phy 499 in. Suppression by red light irradiation of corynespora leaf. Similarly, the phylogenetic analysis of 30 isolates of c. A total of 651 isolates of cucumber corynespora leaf spot fungus corynespora cassiicola collected from cucumber in japan, either with 438 isolates or without 2 isolates a prior history of boscalid use, were tested for their sensitivity to boscalid by using a mycelial growth inhibition method on yba agar medium. Corynespora cassiicola is a species of fungus well known as a plant pathogen. Pdf intraspecific variability of corynespora cassiicola. Pathogenicity of corynespora cassiicola on different hosts in.
This fungus is present in some hevea rubber plantations and nurseries in. It is a reemerge pathogen on cotton in southeastern us. Corynespora cassiicola is causing numerous emerging diseases, including target spot of cotton a disease is emerging if increase in host range, geographic range, or incidence is observed, or new strains evolve. Genomewide analysis of corynespora cassiicola leaf. Although variation in aggressiveness to cotton and tomato were observed, no genetic diversity.
Eco ri, msp i and rsa i endonucleases identified all isolates as corynespora cassiicola. We are setup to allow easy downloads of individual projects and dont provide means to download whole collections of data. Pdf effects of corynespora cassiicola on lantana camara. Corynespora cassiicola appeared to be a minor pathogen for okra. Our raw reads are also published to sra at ncbi for bulk download needs. Arvind kumar kurre, meghchand dewangan and dantre, r. Occurrence of corynespora cassiicola isolates resistant to. Corynespora cassiicola berk and curt wei, incitant of leaf spot of brinjal, survives at the room temperatures 2830 c.
Frontiers compounds released by the biocontrol yeast. Evaluation of fungicides against target leaf spot caused. Sensitivity of corynespora cassiicola from soybean to carbendazim and prothioconazole article pdf available in tropical plant pathology 385. He found that the fungus was pathogenic to leaves of hydrangea, hydrangea macrophylla ser. Two primer pairs showed specificity to corynespora cassiicola and one primer pair showed specificity to. A new record of leaf spot disease on aquilaria malaccensis lamk in india. Gao wei 1,2, li baoju 2, wang wanli 1, hao yongjuan 1, shi yanxia 2. Genome sequences and snp analyses of corynespora cassiicola. A new foliar disease, corynespora leaf spot, has been. Decalactone derivatives from corynespora cassiicola, an. The present study combines the examination of toxins produced by c. Corynespora cassiicola infection is common in plants, but the human corynespora cassiicola infection in our report is rare according to the literature. Corynespora cassiicola is a common plant pathogen that causes leaf spot disease in a broad range of crop, and it heavily affect rubber trees in malaysia hsueh, 2011. Frequency distribution of ec 50 values for tetramycin against 91 corynespora cassiicola isolates obtained from different cities in shandong, as based on a mycelial growth, b spore germination, and c germ tube elongation at several concentrations.
Jsv provided expertise for the manual tuning of clustering parameters. Clonality and geographic structure of hostspecialized. Borah r k, ahmed f s, sarmah g s, bhabesh gogoi, 2012. Pdf symptomatology, hosts, and pathogenicity of corynespora. These climatic conditions would limit the ability of the pathogen to. An indirect phenotyping method was developed in order to estimate the susceptibility of rubber tree clonal varieties to corynespora leaf fall clf disease caused by the ascomycete corynespora cassiicola. No known resistance to target spot is currently available in commercial cultivars.
Pdf corynespora brown spot of papaya corynespora cassiicola. Evaluation of fungicides against target leaf spot caused by corynespora cassiicola. Corynespora cassiicola is a destructive plantpathogenic fungus causing widespread target spot epidemics, including outbreaks on cotton, soybean, and tomato in the southeastern united states. Initial characterization of corynespora cassiicola affecting florida tomatoes. Genetic variation of 42 isolates of corynespora cassiicola, a destructive fungal pathogen of many economically important crop plants including rubber, was. Subcutaneous infection caused by corynespora cassiicola, a. Corynespora cassiicola requires high humidity for infection and is additionally favored in locations with substantial periods of high moisture 1644 hours. Ripe fruits and abaxial surfaces of the leaves were significantly more susceptible to infection than unripe fruits and adaxial surfaces of leaves.
Cotton, cowpea, cucumber, eggplant, sesame, soybean, tobacco. On the lower surface, small, light brownish, somewhat soakedwater lesions appear first. Genetic determinism of sensitivity to corynespora cassiicola. Corynespora cassiicola is a common pathogen of grasses and other herbaceous plants in the tropics 3,4. Corynespora cassiicola from soybean to carbendazim and prothioconazole. Pdf on sep 10, 2012, jose ricardo liberato and others published. Diagnostic features of corynespora cassiicola and its associated diseases identifying c. Frequency distribution of ec 50 values gml for thiophanatemethyl from 40 greenhousecollected isolates of corynespora cassiicola from african violet in 2007 displayed with a maximum xaxis value of 1. Here we confirm the occurrence of this species as an agent of subcutaneous phaeohyphomycosis. Corynespora brown spot of papaya corynespora cassiicola. Corynespora cassiicola was pathogenic on the roots and hypocotyls of soybean seedlings grown in infested soil at ottawa. Sensitivity of corynespora cassiicola from soybean to carbendazim and prothioconazole sheila a. The risk corynespora cassiicola would pose to california is evaluated below.
Pdf chlamydospore formation by corynespora cassiicola. Several lineages of this pathogen have been recognized, but the classification of isolates into clades is timeconsuming and still sometimes leads to. Corynespora cassiicola, which causes corynespora leaf fall disease, is one of the most destructive and economically important fungal pathogens of hevea rubber trees in asian and african countries. Diversity of the cassiicolin gene in corynespora cassiicola and relation with the pathogenicity in hevea brasiliensis. Mar 28, 2017 corynespora cassiicola was consistently isolated from the leaf spots on inoculated lettuce plants following the same method that was used for the initial isolations. Pathogenicity of corynespora cassiicola on soybean canadian.
Infection process of corynespora cassiicola tagged with gfp on hevea brasiliensis article pdf available in australasian plant pathology 435. Genomic insight into pathogenicity of dematiaceous fungus. Suppression by red light irradiation of corynespora leaf spot. Pdf first report of corynespora cassiicola causing leaf and. Differentiation of corynespora cassiicola and cercospora sp. In rest of the cultivars, the occurrence of this fungus was very poor to nil.
A new boll rot disease of cotton gossypium hirsutum l. For your convenience and fast downloads you can use globus and api. Later, the lesions become brown, necrotic, slightly sunken, with brownreddish margins. Download fulltext pdf download fulltext pdf physiological characterization of corynespora cassiicola isolates article pdf available in tropical plant pathology 384.
The yellowish area surrounding the lesion can be seen only against the light. Aug 31, 2018 corynespora cassiicola infection is common in plants, but the human corynespora cassiicola infection in our report is rare according to the literature. Phaeohyphomycosis caused by a plant pathogen, corynespora. Corynespora cassiicola is the causal organism of the most devastating leaf disease of rubber in sri lanka. Sensitivity of corynespora cassiicola from soybean to. Extensive superficial necrotic lesions developed on seedlings from inoculated seeds planted in the field in midmay, but few symptoms appeared on seedlings planted in late may and in june. Mostly known as a necrotrophic plant pathogen, it has also been associated with rare cases of human infection. Continuing to use this website means you agree to our use of cookies. Detection of corynespora cassiicola in soil with realtime quantitative pcr.
The planar structures were determined by extensive nmr spectroscopic data. In other words, the leaf wetness is likely a major environmental factor driving the disease for this fungal pathogen. A necrotic subcutaneous lesions over the dorsal aspects of both hands with purulent discharge in a patient with chronic subcutaneous infection caused by corynespora cassiicola. Growth sensitivity of corynespora cassiicola to thiophanate. However, no polymorphisms were found at the its sequences. The humidity may be used as a surrogate for leaf wetness, as it is an essential component of an environment and has. A new leaf and berry spot disease, and leaf fall caused by corynespora cassiicola is reported on coffea canephora in brazil. Detection of corynespora cassiicola in hevea rubber tree from. Target spot of tomato, caused by corynespora cassiicola, was first reported in florida in 1972. Pdf the fungus corynespora cassiicola is an important pathogen that causes necrotic lesions in several plant species. Unlimited viewing of the articlechapter pdf and any associated supplements and. Cca corynespora blight and target spot corynespora cassiicola gc powdery mildew golovinomyces cichoracearum ccu scab and gummosis cladosporium cucumerinum kgmmv kyuri green mottle mosaic virus cgmmv cucumber green mottle mosaic virus pcu downy mildew pseudoperonospora cubensis.